Coding

Part:BBa_K1319009:Design

Designed by: Michael Osthege   Group: iGEM14_Aachen   (2014-10-02)


mRFP-gal3-his fusion protein CDS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 793
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 555
    Illegal AgeI site found at 667
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The stop codon and barcode sequence was removed from E1010. We assembled the DNA by Gibson assembly, but designed the sequence to contain RFC[25] scars (ACCGGC) between the domains.


Source

it's a combination of other part sequences.

References